Descrição
Plant micropropagation is one of the most important methods for the clonal propagation of Eucalyptus species. This study focused on the in vitro elongation stage of Eucalyptus dunnii. The experiments were conducted at the laboratory of in vitro culture of forest species, department of forestry sciences, federal university of Lavras (UFLA). Eucalyptus seeds were placed on a water–agar medium. After the multiplication phase, during the elongation stage, explants were cultured in three media: control (without zinc), traditional (with zinc sulfate), and nanoparticulate (with zinc oxide). These were subjected to four light conditions: white, blue, red, and purple. Monthly assessments included survival percentage, vegetative bud formation percentage, average number of vegetative buds and leaves, shoot length (cm), percentage and number of roots, root length (cm), oxidation percentage, and contamination percentage. At the end of the second month, the results showed that maximum survival and vegetative bud formation percentage occurred in the nanoparticulate/white treatment (91.66%), while the minimum was observed in control/blue (16.66%). The maximum average number of vegetative buds was found in traditional/blue (17.33) and the minimum in control/red (0.66). The maximum average number of leaves was obtained in traditional/blue (72.08), whereas the minimum was in control/red (2.83). The maximum shoot length (5.87 cm) occurred in nanoparticulate/white, while the minimum (0.25 cm) occurred in control/red. The maximum percentage of root formation was recorded in nanoparticulate/red and traditional/red (66.66%), with the minimum in control/blue (0%). The maximum number of roots was obtained in traditional/red (4.08), and the minimum in control/blue (0). The maximum root length (4.62 cm) was found in nanoparticulate/white, while the minimum (0 cm) was in control/blue. The highest oxidation percentage was observed in nanoparticulate/white and traditional/red (83.33%), and the lowest in control/blue and control/white (8.33%). The maximum contamination percentage was observed in control/blue (83.33%), while the minimum was recorded in traditional/white, traditional/red, nanoparticulate/white, and nanoparticulate/red (25%). The application of nanotechnology may promote advances in the in vitro cultivation of Eucalyptus dunnii. The authors would like to thank IPEF, CNPq, CAPES, and FAPEMIG.
Keywords: Eucalyptus dunnii, In vitro culture, Micropropagation, Nanotechnology, Zinc oxide, Zinc sulfate.
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